International Journal of STD & AIDS > Volume 13, Number 3 > Pp. 171-173

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Original Articles

Evaluation of a rapid test for the detection of antibodies to human immunodeficiency virus type 1 and 2 in the setting of multiple transmitted viral subtypes

Steven J Reynolds, Lubaki M Ndongala, Chi-Cheng Luo, Kashamuka Mwandagalirwa, Atibu J Losoma, Kasali J Mwamba, Edidi Bazepeyo, Nzila E Nzilambi, Thomas C Quinn and Robert C Bollinger

Division of Infectious Diseases, Department of Medicine, Johns Hopkins University, Ross Building, Room 1150, 720 Rutland Avenue, Baltimore, MD 21205, USA; Division of Infectious Diseases, Department of Medicine, Johns Hopkins University, Ross Building, Room 1150, 720 Rutland Avenue, Baltimore, MD 21205, USA; Centers for Disease Control and Prevention, Atlanta, USA; Division of Infectious Diseases, Department of Medicine, Johns Hopkins University, Ross Building, Room 1150, 720 Rutland Avenue, Baltimore, MD 21205, USA; Projet SIDA, Hôpital Général de Kinshasa, The Democratic Republic of Congo; Projet SIDA, Hôpital Général de Kinshasa, The Democratic Republic of Congo; Projet SIDA, Hôpital Général de Kinshasa, The Democratic Republic of Congo; Projet SIDA, Hôpital Général de Kinshasa, The Democratic Republic of Congo; Division of Infectious Diseases, Department of Medicine, Johns Hopkins University, Ross Building, Room 1150, 720 Rutland Avenue, Baltimore, MD 21205, USA; Division of Infectious Diseases, Department of Medicine, Johns Hopkins University, Ross Building, Room 1150, 720 Rutland Avenue, Baltimore, MD 21205, USA

Rapid HIV testing is an appealing strategy in the approach to HIV diagnosis in developing countries. Concern has been raised about the use of these tests in the setting of multiply transmitted HIV subtypes. We sought to compare the OraQuick^TM HIV-1/2 Test, a qualitative immunochromatographic test for the detection of antibodies to HIV-1 and HIV-2 using stored sera, with a conventional enzyme immunoassay (EIA)/Western blot (WB) algorithm. The study design used was a blinded retrospective study. Samples were collected on patients attending sexually transmitted disease clinics and tuberculosis clinics in Kinshasa, the Democratic Republic of Congo and included 72 known HIV seropositive and 131 known HIV seronegative subjects. All 72 known HIV seropositive samples were positive by OraQuick and all 131 known HIV seronegative samples were negative by OraQuick resulting in 100% sensitivity and specificity. We conclude that the OraQuick rapid HIV-1/2 test performs well in the setting of diverse HIV viral subtypes.

Key Words: HIV DIAGNOSTIC TESTS • HIV SUBTYPES

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